Journal: Scientific Reports

Article Title: Three Dimensional Glomerular Reconstruction: A Novel Approach to Evaluate Renal Microanatomy in Diabetic Kidney Disease

doi: 10.1038/s41598-019-38646-z

Figure Lengend Snippet: Mesangial area quantification using Transmission Electron Microscopy (TEM) and Point Sample Intercept (PSI) methods. ( a ) Mesangial area and estimated volume was calculated from TEM images (Scale bar 4 µm). Images were analysed (Digimizer 4.2.2) and mesangial area (red) was identified by an expert operator. Transverse sections through the middle of representative glomeruli were chosen and all identifiable mesangial area per glomerulus was measured. The mean mesangial area in a glomerulus per subject was then calculated. ( b ) Fractional volumes of glomerular components were calculated using PSI method. Using the Cavalieri’s method of segmentation combined with automated point counting (yellow) the mesangial volume was estimated (Fiji by Image J 1.51e) (Scale bar 50 µm).

Article Snippet: EM assessment was performed using computer-assisted measurement through Digimizer (version 4.2.2) image analysis software (MedCalc Software, Ostend, Belgium).

Techniques: Transmission Assay, Electron Microscopy